From: João Ribeiro (jribeiro_at_ks.uiuc.edu)
Date: Wed Jun 13 2018 - 09:24:26 CDT

Hi Ritwika,

could you please specify the exact steps that you took? Only then we can
help you debug what is wrong with the mutation process.

Thank you

Best

João

On Mon, Jun 11, 2018 at 12:23 AM Ashar Malik <asharjm_at_gmail.com> wrote:

> Have you tried using Modeller.
> https://salilab.org/modeller/wiki/Mutate%20model
>
> The script provided in the above example is simple to use. You give it the
> protein name (same as file name except the ".pdb" part), the residue
> position to mutate, the residue name to mutate to and the chain and that's
> it. Line 13 in the script gives more details on the usage of the script.
>
> On Mon, Jun 11, 2018 at 9:51 AM Bose, Ritwika <ritwika.bose_at_richmond.edu>
> wrote:
>
>> Hello,
>> I am a student at University of Richmond, working with a Virus like
>> particle bacteriophage. I was trying to mutate one of the residues on the
>> monomer to another, but despite following all the steps, the software
>> generates the MUTATED file without any changes to the intended amino acid
>> at all. The monomer consists of 3 chains and I wondered if that could be an
>> issue. I would appreciate any help on this matter.
>> Thank you,
>> Ritwika Bose
>>
>
>
> --
> ……………………………………………………...
> João Vieira Ribeiro
> Theoretical and Computational Biophysics Group
> Beckman Institute, University of Illinois
> http://www.ks.uiuc.edu/~jribeiro/
> jribeiro_at_ks.uiuc.edu
> +1 (217) 3005851
>