From: Vince Cho (vince_at_fastmail.fm)
Date: Tue Jul 03 2012 - 12:44:39 CDT

Hello Sebastián,

Thanks for pointing that out. Actually I just realized that too. Just
to make things clear for other users, the number I mentioned was for
building a protein-membrane mix.

But anyway, I skimmed through all 34 lipids and still couldn't find one
I need which has a single tail - most of these 34 lipids seemed to have
double tails, correct me if I'm wrong.

That said, do you think there's a way to utilise the protocol of
CHARMM-GUI membrane builder for building a membrane of single-tail lipid
molecules? I was thinking of adopting the distance between neighbouring
lipid molecules, but I guess they might be quite different for single-
and double-tailed lipids, no?

V

On 07/03/2012 11:31 AM, Sebastián Gutiérrez wrote:
> Hello Vince,
>
> are you sure about what you said regarding CHARMM-GUI?
> Last time i checked there were 34 different lipids available (plus
> cholesterol) for membrane building.
> Check this link:
> http://charmm-gui.org/?doc=input/membrane_only&step=1
> <http://charmm-gui.org/?doc=input/membrane_only&step=1>
>
> You can also build heterogeneous membranes with this tool.
>
>
> Best regards,
>
> Sebastián
>
> On Thu, Jun 28, 2012 at 12:03 AM, Vince Cho <vince_at_fastmail.fm
> <mailto:vince_at_fastmail.fm>> wrote:
>
> Hello All,
>
> I have a quick question about building a membrane in general.
> Apparently, the "membrane builder" module in VMD offers only 2
> types of lipid. CHARMM-GUI offers a bit more, but that's limited
> to 6 as well.
>
> So, if I want to build a membrane of an arbitrary lipid, what
> would be the best way to proceed?
>
> The quick and dirty method would be to start from a PDB file of a
> single molecule and make an array of it, but I feel like I will
> encounter a lot of bugs this way. Is this how the Membrane
> Builder module works?
>
> Thanks,
>
> V
>
>