Mechanisms of Protein Synthesis by the Ribosome

See also the websites on the Dynamics of Protein Translocation and Molecular Dynamics Flexible Fitting (MDFF).

Introduction

The translation of genetic information into proteins is essential for life. At the core of this process lies the ribosome, a quintessential large (2.5-4.5 MDa) molecular machine responsible for translating genetic material into functional proteins. In a growing cell, ribosomes comprise up to half of the net dry weight. Because of its fundamental role in the cell, 50% of all efforts to develop antibiotics target bacterial ribosomes, taking advantage of the structural differences between bacterial and human ribosomes.

The structure and function of the ribosome are fascinatingly complex. Two-thirds of the ribosome consist of ribosomal RNA (rRNA), while over 50 ribosomal proteins make up the rest. The genetic information is delivered to the ribosome by a messenger RNA (mRNA). Transfer RNAs (tRNAs) are adapter molecules, each equipped with an anticodon to match the codons in the mRNA, and charged with an amino acid that corresponds to the anticodon as dictated by the genetic code. The ribosome contains three tRNA-binding sites: A, P, and E (see elongation cycle box, or watch a movie). In addition to mRNA and tRNAs, the ribosome interacts with protein factors such as the elongation factors Tu (EF-Tu) and G (EF-G), that are important players in the so-called elongation cycle. The elongation cycle results in the addition of an amino acid to the nascent peptide chain, and consists of three main steps. In the decoding step, a ternary complex comprised of an aminoacyl-tRNA (aa-tRNA), EF-Tu, and GTP binds to the ribosome, leading to the recognition of the codon by the anticodon. The following step is the peptidyl transfer. Here the peptide chain bound to the P-site tRNA is covalently linked to the amino acid bound to the A-site tRNA. In the translocation step, the position of the mRNA/tRNA complex shifts by one codon, accompanied by a ratchet-like motion of the ribosomal subunits.

Elongation Cycle Fig 1. Elongation cycle of protein synthesis. The ribosome is shown in top view, with the small subunit (transparent yellow) below the large subunit (transparent blue). (i) The ribosome in the pre-translocational state with tRNAs in the A (magenta) and P (green) sites. After spontaneous peptidyl transfer, the nascent peptide is covalently attached to the A-site tRNA. (ii) The elongation factor EF-G in complex with GTP (blue) has bound to the ribosome to facilitate the translocation of tRNAs to the P (green) and E (yellow) sites. The translocation is induced by GTP hydrolysis accompanied by large transient conformational changes in the EF-G and the ribosome. (iii) The release of the EF-G after GTP hydrolysis leaves the ribosome in the post-translocational state. It is now ready to accept a new aminoacyl-tRNA (white) presented to the ribosome by the ternary complex, comprising, in addition to the new aminoacyl-tRNA, the the elongation factor EF-Tu and a GTP (red). (iv) The ribosome with a bound ternary complex (magenta and red) in place. It has been suggested that when the ternary complex binds to the ribosome, the E-site tRNA moves further away from the P site to the so-called E2 site (orange). The snapshot shown here is part of the decoding step, where the aminoacyl-tRNA whose anticodon matches the next codon in the mRNA is selected to enter the A site, accompanied by GTP hydrolysis and conformational changes. In case of a match, EF-Tu with the hydrolyzed GTP and the E-site tRNA leave the ribosome, leaving it in the pre-translocational state (i). This figure is adapted from Frank, J. in Conformational Proteomics of Macromolecular Architechtures. World Scientific Publishing Comp. Singapore, 2004. Watch a movie of this process.


Due to great advances in the structural resolution of the ribosome, an impressive feat given its large size, the system is considered one of the hottest focal areas in molecular cell biology today. During the process of translation, the ribosome undergoes several conformational changes and binds to different factors that catalyze specific reactions. As detailed below, techniques to determine structure of the ribosome can only image snapshots of the ribosome, often at medium to low resolution. Atomic details of the interactions between the factors and the ribosome, along with a dynamic description of the conformational changes of the ribosome itself, are crucial to understanding its function.

Our goal is to provide a complete description of the structural dynamics of translation using computational biology. Eventually, we would like to be able to show you an animation much like this beautiful illustrative movie but with every frame based on physically realistic simulations that incorporate experimental data.

Obtaining atomic models of the ribosome at functional states

Detail of MDFF of EF-Tu bound to the ribosome

Fig. 2. Detail of a fitted atomic model into the EM density using MDFF.

Due to its sheer size and complexity, the ribosome presents an outstanding challenge for traditional methods for high-resolution structure determination such as X-ray crystallography and nuclear magnetic resonance spectroscopy. X-ray crystallographers have conquered this challenge: today, the Protein Data Bank has several structures of entire ribosomes from different laboratories. However, these structures remain difficult to obtain for factor-bound ribosomes, which are key to understand the dynamics of translation.

Cryo-electron microscopy (cryo-EM) provides an alternative to obtain structural information of the ribosome in many different functional states. Cryo-EM produces three-dimensional density maps of ribosomes at medium resolution (7-12Å) without the need to purify large amounts of sample or to form highly ordered crystals, both required in X-ray crystallography. This technique thus can capture the ribosome at different conformational states, as shown in Fig. 1. However, these maps don't reach atomic resolution, needed to understand the function of the ribosome in detail along its functional cycle.

In order to reach atomic resolution, we have developed a technique called molecular dynamics flexible fitting (MDFF) to morph atomic-resolution structures emerging from X-ray crystallography into cryo-EM maps. Details of the MDFF method can be found here. Fig. 2 shows a detail of an atomic model flexibly fitted into a cryo-EM map of the ribosome.

We have teamed with the laboratories of Joachim Frank and Roland Beckmann, experts on ribosome structure determination by cryo-EM, to study the dynamics of the ribosome.

How does the ribosome induce the GTPase activity of EF-Tu?

Atomic structure fitted to an EM map of the ribosome

Fig. 3. Atomic model of the ribosome bound to factors derived from a 6.7-Å EM map. The EM data is shown in transparent surface, with atomic model generated with MDFF showed in cartoon.

During elongation, the mRNA-programmed ribosome is presented with an amino acid by a tRNA in complex with elongation factor Tu (EF-Tu) and GTP. The tRNA assumes the bent A/T position, storing elastic energy. If the ribosome recognizes the right codon-anticodon interaction, GTPase activity in EF-Tu is stimulated over 75Å away. How the ribosome induces GTP hydrolysis has been subject of intensive study. We now know that GTP hydrolysis takes place through an in-line attack on the gamma phosphate by a water molecule, and that His84 is the catalytic residue.

Atomic model of EF-Tu from cryo-EM

Fig. 4. Atomic model of a ribosome-bound EF-Tu. The switch regions are highlighted: Switch I (Sw1) in blue, Switch II (Sw2) in orange, and P-loop in green.

In order to understand GTP hydrolysis in EF-Tu, we studied the ribosome-induced EF-Tu conformational changes that trigger GTP hydrolysis on the factor as revealed by cryo-electron microscopy (cryo-EM) studies. We applied MDFF to obtain an atomic model of a 6.7-Å cryo-EM map of the pre-accommodated E. coli 70S ribosome bound to the Phe-tRNAPhe:EF-Tu:GDP ternary complex stalled by the antibiotic kirromycin (kir), showed in Fig. 3.

EF-Tu has three important regions that play a prominent role in its GTPase activity: Switch I (EF-Tu residues 40--62; E. coli numbering) Switch II (80--100) and P-loop (18--23). All of these regions undergo characteristic conformational changes in EF-Tu. Crystal structures of EF-Tu in the GTP form display a "hydrophobic gate" formed by residues Val20 (in the P-loop) and Ile60 (In Switch I) that controls access to the GTP binding pocket. A schematic representation of this gate is shown in Fig 5A, the crystal structure shown in Fig. 5B. His84 (in Switch II) needs to enter through this gate to perform its catalytic role. Thus, this gate must be opened when the right codon-anticodon interaction is recognized by the ribosome. A crystal structure of EF-Tu outside the ribosome, but bound to the antibiotic aurodox that is thought to simulate interaction with the ribosome, displays an open gate (Fig. 5D). This antibiotic binds to EF-Tu and stimulates GTPase activity, preventing EF-Tu from binding the ribosome, and therefore preventing translation and killing the cell. The fact that an open gate is found in the case of enhanced GTPase activity hints that this mechanism is used by the ribosome as well.

Hydrophobic gate of EF-Tu

Fig. 5. Hydrophobic gate of EF-Tu. (A) Schematic representation of the gate. (B) The closed gate revealed by an X-ray structure of EF-Tu free from the ribosome (PDB 1OB2). (C) The open gate revealed by MDFF (PDB 3FIH). (D) The open gate present in an antibiotic-bound X-ray structure (PDB 1HA3).



But how does the ribosome open this hydrophobic gate? Let's look at each wing of the gate:

Interaction between the P-loop and SRL

Interaction between the P-loop and SRL

Fig. 6. Interaction between the P-loop (green) and the SRL in the 50S (blue).
One wing of the hydrophobic gate is formed by residue Val20. The MDFF model reveals that the P-loop interacts with the sarcin-ricin loop (SRL; nucleotides 2646--2674 of the 23S rRNA). Specifically, the interaction occurs between His19 of EF-Tu and A2660 in the tetraloop of SRL. This contact with SRL stabilizes the P-loop in its position, acting as an anchor point for this wing of the gate, while the other wing opens as described below.

Interaction between Switch I and the 16S rRNA

The other wing of the hydrophobic gate (Ile60) is part of Switch I, which due to its flexibility sometimes is difficult to observe in structural studies. Our data and analysis reveal that Switch I interacts with the junction formed by helices h8 and h14 of the 16S rRNA (Fig. 7A,B). This interaction involves an opening of the hydrophobic gate, as shown in Fig. 5C. The contact between Switch I and the 16S rRNA h8/h14 junction could serve as a pathway for signal transduction to/from the decoding center through helix h44 of the 16S rRNA; this helix interacts with the decoding center at one end and with the h8/h14 junction at the other (Fig. 7C).

Interaction between the Switch I and the 16S rRNA

Fig. 7. Interaction between the Switch I of EF-Tu and the 16S rRNA. (A) Stereo view of the different conformations of Switch I when bound (blue) and unbound (lime) to the ribosome. The EM map is shown in gray mesh. (B) Detail of the interaction. Elements of the ribosome-bound EF-Tu (red and blue) and the 30S (yellow) and 50S (cyan) subunits are shown along with the crystal structure of the Switch I in the ribosome-unbound EF-Tu (lime; PDB 1OB2). (C) Overview of the ternary complex (EF-Tu in red, tRNA in purple) bound to the 30S subunit (yellow).

Open Sesame

Once the gate is opened, the side chain of His84 can reach the GTP binding pocket and activate hydrolysis, as shown in Fig. 5C; note the similarity to the conformation seen in the aurodox-bound structure of EF-Tu outside of the ribosome, shown in Fig. 5D.

EM Map and Atomic Models

The atomic coordinates have been deposited in the Protein Data Bank, with PDB ID codes 3FIH (30S and factors) and 3FIK (50S). The cryo-EM density map has been deposited to the 3D-EM database , with code EMD-5036.

Get all the files and a VMD saved state to visualize them together here (107 Mb).

Nascent-chain mediated translational stalling

Certain nascent peptide chains are able to regulate ribosome function while they are still being synthesized, i.e., when they are still inside the ribosomal exit tunnel. One of the classical examples is TnaC, a leader peptide of the tryptophanase operon in E. coli. At high concentrations of tryptophan, TnaC stalls the ribosome, inhibiting termination of its synthesis. Through an intricate gene regulatory mechanism, stalling ultimately leads to the expression of genes responsible for degrading tryptophan.

The structural basis for TnaC-mediated translational stalling was addressed by obtaining a 5.8-Å cryo-EM map of the ribosome stalled by TnaC and high concentrations of tryptophan (Fig. 8). The cryo-EM data shows that the nascent chain adopts a distinct conformation in the exit tunnel. We applied MDFF to obtain an atomic model of the entire ribosome and the stalling nascent chain (Fig. 8F). The model allowed us to map the contacts between TnaC and the exit tunnel, as well as propose possible communication pathways that would lead to inactivation of the catalytic center of the ribosome (the so-called peptidyltransferase center, or PTC). One of the main findings was that two critical ribosomal residues at the PTC adopt conformations that are incompatible with cohabitation by release factors, which catalyze termination of protein synthesis.

Cryo-EM structure of the 70S:TnaC complex

Fig. 8. Cryo-EM reconstruction of the TnaC:70S complex. (A and B) Cryo-EM reconstruction of the control E. coli 70S ribosome at 6.6-Å resolution. (C and D) The 5.8-Å density of the 70S:TnaC complex. (E) Isolated density for mRNA, tRNA, and nascent chain, with the positions of selected ribosomal proteins shown. (F) Same as (E), but with the atomic model obtained using MDFF shown within the transparent density.

Recognition of the regulatory nascent chain TnaC by the ribosome

The atomic model of the ribosome stalled by TnaC, presented above, revealed the mechanism of translational arrest. Due to the limited resolution, however, the TnaC side chains and their interactions with the ribosome could not be unambiguously visualized. In fact, 10 possible model of the TnaC nascent chain inside the ribosome were obtained (Fig. 9, left). In order to characterize the precise interactions that allow the ribosome to specifically recognize the TnaC sequence, we performed extensive molecular dynamics (MD) simulations of each of the 10 different models of TnaC inside the exit tunnel for a total aggregate time of over 2 microseconds.

The simulations allowed us to characterize the precise interactions that allow the ribosome to recognize the TnaC sequence inside the exit tunnel (Fig. 9, right). The critical TnaC residue Trp12 is recognized via a cation-pi interaction by Arg92 of ribosomal protein L22. Since classical MD force fields fail to accurately describe such interactions, the study was complemented by quantum chemistry calculations. The MD simulations also revealed that Asp16, another critical TnaC residue, is recognized by Lys90 of L22, also essential for stalling. Moreover, bioinformatic analyses of naturally occurring proteins that share the TnaC consensus sequence suggested mutations that may alleviate TnaC-mediated stalling.

Structure of TnaC inside the exit tunnel

Recognition of TnaC by the ribosome

Fig. 9. (Left) Structure of TnaC inside the exit tunnel. Shown are 10 different TnaC models with critical residues highlighted. Also shown are ribosomal residues with available biochemical data regarding TnaC-mediated stalling. Click the image to see a movie of a rotating structure (size: 22 M). (Right) Recognition of critical TnaC residues by the ribosome reveal by extensive molecular dynamics simulations. Shown is a cation-pi interaction between critical residue Trp12 (TnaC) and Arg92 (ribosomal protein L22), as well as a salt bridge between critical residues Asp16 (TnaC) and Lys90 (L22).

How does the L1 stalk interact with tRNAs in hybrid state?

After the accommodation of the tRNA brought by EF-Tu and successful peptidyl transfer, the ribosome is able to spontaneously undergo an intersubunit rotation (Fig. 1 (i) to (ii)). In this state the tRNAs assume a hybrid occupation relative to the ribosome: while the anticodon is still located in the original site on the small subunit, the other end of the tRNA is shifted to the next binding site on the large subunit generating so called A/P and P/E hybrid states. The P/E tRNA engages in interactions with the L1 stalk, a very dynamic part of the ribosome. These interactions are thought to stabilize the hybrid state. Interestingly, based on FRET experiments it seems that the initiator tRNA interacts weaker with the L1 stalk.

Using MDFF and equilibrium MD we addressed the nature of the interactions between the L1 stalk and a tRNA in the hybrid P/E state. In particular, we compared the behavior of the initiator tRNAfMet versus the elongator tRNAPhe. Not only do the two tRNAs assume different conformations within the ribosome (Fig. 10), they interact differently with the L1 stalk. While for both tRNAs a peculiar stacking with the ribosomal RNA of the L1 stalk is observed, it is less pronounced for the initiator tRNA compared to an elongator tRNA (Fig. 10). Interestingly, the behavior of the tRNAs is strongly impacted by their respective modification patterns.

Structure of P/E tRNAs within the ribosome

Recognition of TnaC by the ribosome

Fig. 10. (Left) Superimposed structures of tRNAPhe and tRNAfMet within the ribosome. (Center) The interactions between tRNAPhe and the L1 stalk. The stacking interactions are shown as surfaces within the dotted box. (Right) The corresponding interactions for tRNAfMet.

What is more, the simulations show that one of the domains of the ribosomal protein L1 can detach from the ribosomal RNA and move more or less independent (see movie). However, the physiological implications of this behavior are not clear, but future FRET experiments could shed light on a possible function.

Translocation of tRNAs inside the ribosome

During the spontaneous intersubunit rotation, or the so-called ratcheting, of the ribosome, tRNAs inside the ribosome adopt two different conformations, the classical (A/A and P/P) state and the hybrid (A/P and P/E) state. Together with the motion of L1 stalk, these conformations are collectively termed as two distinct states, namely "macrostate I" (unratcheted ribosome, classical tRNAs and open L1 stalk) and "macrostate II" (ratcheted ribosome, hybrid tRNAs and a closed L1 stalk), as shown in Fig 11A. The transition from "macrostate I" to "macrostate II" is essential for translocating the tRNAs inside the ribosome such that the site for tRNA bearing the next amino acid can be vacated. The existence of intermediate states in between these two conformations is of great debate due to lack of structural data.

Application of MDFF to cryo-EM data of the ribosome revealed two previously unseen intermediate states (Fig 11B). The identification of these new intermediate states help elucidating the pathway of transition, in particular the formation of hybrid tRNAs inside the ribosome. Our results also support the idea that the ribosome is employing a Brownian mechanism to progress through the distinct states during the translocation of mRNA-tRNAs.

Intermediate states in mRNA-tRNA translocation

Fig. 11. (A) Two distinct states of ribosomal complex during translocation of mRNA-tRNA. Macrostate I has an unratcheted ribosome, classical tRNAs (orange and green) and an open L1 stalk (purple). Macrostate II has a ratcheted ribosome, hybrid tRNAs and closed L1 stalk. (B) Two new intermediates (in dashed-lined box) identified by cryo-EM analysis and MDFF.

Stalling of the ribosome by SecM

Similar to TnaC described above, the peptide SecM exists solely to stall the ribosome synthesizing it. But unlike TnaC, which also requires the presence of high levels of trytophan, SecM has an intrinsic stalling capability. Stalling of the ribosome synthesizing SecM provides time for a downstream RNA helix on the same mRNA strand to unwind. Unwinding of this helix then allows for a new ribosome to bind and synthesize a new protein, SecA, a bacterial ATP-driven translocase that aids the passage of nascent proteins across membranes in conjunction with SecY (see also Dynamics of Protein Translocation). When sufficient levels of SecA have been reached, SecA interacts with the SecM-stalled ribosome to pull on SecM, freeing it and allowing translation to resume (illustrated schematically in Fig. 13). SecM, which serves no other purpose than to stall the ribosome, is released into the cell and degraded.

Much of what is known about SecM stalling comes from biochemical experiments, in which every amino acid in SecM's sequence has been mutated and the resulting effects measured. These experiments revealed few residues as critical, although one stands out in every species as invariable: arginine 163 (R163, see Fig. 13 below). However, until recently, little was known about the precise mechanisms at work. A cryo-EM map of a SecM-stalled ribosome revealed a shifted linkage in the PTC between the P-site tRNA and the SecM peptide. Although the shift was only 0.2 nm, it was hypothesized to be sufficient to inhibit peptide-bond formation, preventing synthesis of the remainder of SecM.

In order to elucidate precisely how SecM stalls the ribosome, we first applied MDFF to the cryo-EM map of the SecM-stalled ribosome to develop an atomic model of the complex. Next, we carried out extensive simulations of this wild-type model along with mutants of both the ribosome and SecM known to disrupt stalling. We discovered a putative communication pathway between the critical R163 and the PTC that causes the observed shifted linkage; the pathway is shown schematically in Fig. 13. Finally, through steered MD simulations in which we pulled on the N-terminal end of SecM outside the ribosome, we determined that the pulling action of SecA can break the crucial interactions identified in our previous simulations and, thus, alleviate stalling.

SecM

Fig. 13. (left) Structure of the SecM-stalled ribosome determined with MDFF. SecA is shown for visualization purposes only. (middle) Atomic-scale view of SecM in the ribosome's exit tunnel, near the PTC at the top. Residues involved in ribosome-SecM interactions are labeled. (right) Schematic of the communication pathway connecting SecM to the ribosome's PTC.

Publications

Publications Database

Ribosome-induced changes in elongation factor Tu conformation control GTP hydrolysis. Elizabeth Villa, Jayati Sengupta, Leonardo G. Trabuco, Jamie LeBarron, William T. Baxter, Tanvir R. Shaikh, Robert A. Grassucci, Poul Nissen, Måns Ehrenberg, Klaus Schulten, and Joachim Frank. Proceedings of the National Academy of Sciences, USA, 106:1063-1068, 2009.

Publications Database

Regulation of the protein-conducting channel by a bound ribosome. James Gumbart, Leonardo G. Trabuco, Eduard Schreiner, Elizabeth Villa, and Klaus Schulten. Structure, 17:1453-1464, 2009.

Publications Database

Structural insight into nascent polypeptide chain-mediated translational stalling. Birgit Seidelt, C. Axel Innis, Daniel N. Wilson, Marco Gartmann, Jean-Paul Armache, Elizabeth Villa, Leonardo G. Trabuco, Thomas Becker, Thorsten Mielke, Klaus Schulten, Thomas A. Steitz, and Roland Beckmann. Science, 326:1412-1415, 2009.

Publications Database

Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome. Thomas Becker, Shashi Bhushan, Alexander Jarasch, Jean-Paul Armache, Soledad Funes, Fabrice Jossinet, James Gumbart, Thorsten Mielke, Otto Berninghausen, Klaus Schulten, Eric Westhof, Reid Gilmore, Elisabet C. Mandon, and Roland Beckmann. Science, 326:1369-1373, 2009.

Publications Database

Recognition of the regulatory nascent chain TnaC by the ribosome. Leonardo G. Trabuco, Christopher B Harrison, Eduard Schreiner, and Klaus Schulten. Structure, 18:627-637, 2010.

Publications Database

The role of L1 stalk-tRNA interaction in the ribosome elongation cycle. Leonardo G. Trabuco, Eduard Schreiner, John Eargle, Peter Cornish, Taekjip Ha, Zaida Luthey-Schulten, and Klaus Schulten. Journal of Molecular Biology, 402:741-760, 2010.

Publications Database

Cryo-EM structure of the ribosome-SecYE complex in the membrane environment. Jens Frauenfeld, James Gumbart, Eli O. van der Sluis, Soledad Funes, Marco Gartmann, Birgitta Beatrix, Thorsten Mielke, Otto Berninghausen, Thomas Becker, Klaus Schulten, and Roland Beckmann. Nature Structural & Molecular Biology, 18:614-621, 2011.

Publications Database

Molecular dynamics of EF-G during translocation. Wen Li, Leonardo G. Trabuco, Klaus Schulten, and Joachim Frank. PROTEINS: Structure, Function, and Bioinformatics, 79:1478-1486, 2011.

Publications Database

Structural insights into cognate vs. near-cognate discrimination during decoding. Xabier Agirrezabala, Eduard Schreiner, Leonardo G. Trabuco, Jianlin Lei, Rodrigo F. Ortiz-Meoz, Klaus Schulten, Rachel Green, and Joachim Frank. EMBO Journal, 30:1497-1507, 2011.

Publications Database

Structural characterization of mRNA-tRNA translocation intermediates. Xabier Agirrezabala, Hstau Liao, Eduard Schreiner, Jie Fu, Rodrigo Ortiz-Meoz, Klaus Schulten, Rachel Green, and Joachim Frank. Proceedings of the National Academy of Sciences, USA, 109:6094-6099, 2012.

Publications Database

Mechanisms of SecM-mediated stalling in the ribosome. James Gumbart, Eduard Schreiner, Daniel N. Wilson, Roland Beckmann, and Klaus Schulten. Biophysical Journal, 103:331-341, 2012.

Investigators

Page created and maintained by Elizabeth Villa, Leonardo Trabuco, Eduard Schreiner, and Kwok-Yan Chan.

The contents of this website were reproduced in part with permission from Publications Database

Ribosome-induced changes in elongation factor Tu conformation control GTP hydrolysis. Elizabeth Villa, Jayati Sengupta, Leonardo G. Trabuco, Jamie LeBarron, William T. Baxter, Tanvir R. Shaikh, Robert A. Grassucci, Poul Nissen, Måns Ehrenberg, Klaus Schulten, and Joachim Frank. Proceedings of the National Academy of Sciences, USA, 106:1063-1068, 2009.

Publications Database

Structural insight into nascent polypeptide chain-mediated translational stalling. Birgit Seidelt, C. Axel Innis, Daniel N. Wilson, Marco Gartmann, Jean-Paul Armache, Elizabeth Villa, Leonardo G. Trabuco, Thomas Becker, Thorsten Mielke, Klaus Schulten, Thomas A. Steitz, and Roland Beckmann. Science, 326:1412-1415, 2009.

Publications Database

Recognition of the regulatory nascent chain TnaC by the ribosome. Leonardo G. Trabuco, Christopher B Harrison, Eduard Schreiner, and Klaus Schulten. Structure, 18:627-637, 2010.

Publications Database

The role of L1 stalk-tRNA interaction in the ribosome elongation cycle. Leonardo G. Trabuco, Eduard Schreiner, John Eargle, Peter Cornish, Taekjip Ha, Zaida Luthey-Schulten, and Klaus Schulten. Journal of Molecular Biology, 402:741-760, 2010.

Publications Database

Structural characterization of mRNA-tRNA translocation intermediates. Xabier Agirrezabala, Hstau Liao, Eduard Schreiner, Jie Fu, Rodrigo Ortiz-Meoz, Klaus Schulten, Rachel Green, and Joachim Frank. Proceedings of the National Academy of Sciences, USA, 109:6094-6099, 2012.