Paper Citing NAMD - Abstract
Alberto Batista-Garcia, Ramon; Sanchez-Reyes, Ayixon; Millan-Pacheco, Cesar; Manuel Gonzalez-Zuniga, Victor; Juarez, Soledad; Luis Folch-Mallol, Jorge; Pastor, Nina
A novel TctA citrate transporter from an activated sludge metagenome: Structural and mechanistic predictions for the TTT family
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 82:1756-1764, SEP 2014
We isolated a putative citrate transporter of the tripartite tricarboxylate transporter (TTT) class from a metagenomic library of activated sludge from a sewage treatment plant. The transporter, dubbed TctA_ar, shares similar to 50% sequence identity with TctA of Comamonas testosteroni (TctA_ct) and other beta-Proteobacteria, and contains two 20-amino acid repeat signature sequences, considered a hallmark of this particular transporter class. The structures for both TctA-ar and TctA-ct were modeled with I-TASSER and two possible structures for this transporter family were proposed. Docking assays with citrate resulted in the corresponding sets of proposed critical residues for function. These models suggest functions for the 20-amino acid repeats in the context of the two different architectures. This constitutes the first attempt at structure modeling of the TTT family, to the best of our knowledge, and could aid functional understanding of this little-studies family.
