Abad, Enrique; Rommel, Judith B.; Kaestner, Johannes
Reaction Mechanism of the Bicopper Enzyme Peptidylglycine alpha-Hydroxylating Monooxygenase
JOURNAL OF BIOLOGICAL CHEMISTRY, 289:13726-13738, MAY 16 2014

Background: Peptidylglycine -hydroxylating monooxygenase catalyzes the generation of C-terminal carboxamides of peptide hormones, neurotransmitters, and growth factors for biological activation. Results: We compare several possible reaction mechanisms by means of quantum mechanics/molecular mechanics calculations. Conclusion: Our results suggest that the most likely abstracting species is [CuOOH](2+). Significance: Our computational study proposes a new mechanism, which explains the experimental observations. Peptidylglycine -hydroxylating monooxygenase is a noninteracting bicopper enzyme that stereospecifically hydroxylates the terminal glycine of small peptides for its later amidation. Neuroendocrine messengers, such as oxytocin, rely on the biological activity of this enzyme. Each catalytic turnover requires one oxygen molecule, two protons from the solvent, and two electrons. Despite this enzyme having been widely studied, a consensus on the reaction mechanism has not yet been found. Experiments and theoretical studies favor a pro-S abstraction of a hydrogen atom followed by the rebinding of an OH group. However, several hydrogen-abstracting species have been postulated; because two protons are consumed during the reaction, several protonation states are available. An electron transfer between the copper atoms could play a crucial role for the catalysis as well. This leads to six possible abstracting species. In this study, we compare them on equal footing. We perform quantum mechanics/molecular mechanics calculations, considering the glycine hydrogen abstraction. Our results suggest that the most likely mechanism is a protonation of the abstracting species before the hydrogen abstraction and another protonation as well as a reduction before OH rebinding.

DOI:10.1074/jbc.M114.558494

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