Paper Citing NAMD - Abstract
Zhang, Kai; Zhao, Wei-Jie; Leng, Xiao-Yao; Wang, Sha; Yao, Ke; Yan, Yong-Bin
The importance of the last strand at the C-terminus in beta B2-crystallin stability and assembly
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 1842:44-55, JAN 2014
Congenital cataract is the leading cause of childhood blindness worldwide. Investigations of the effects of inherited mutations on protein structure and function not only help us to understand the molecular mechanisms underlying congenital hereditary cataract, but also facilitate the study of complicated cataract and non-lens abnormities caused by lens-specific genes. In this research, we studied the effects of the V187M, V187E and R188H mutations onSB2-crystallin structure and stability using a combination of biophysical, cellular and molecular dynamic simulation analysis. Both V187 and R188 are located at the last strand of SB2-crystallin Greek-key motif 4. All of the three mutations promoted 3B2-crystallin aggregation in vitro and at the cellular level. These three mutations affected 3B2-crystallin quite differentially: VI 87M influenced the hydrophobic core of the Cterminal domain, VI 87E was a Greek-key motif breaker with the disruption of the backbone H-bonding network, while R188H perturbed the dynamic oligomeric equilibrium by dissociating the dimer and stabilizing the tetramer. Our results highlighted the importance of the last strand in the structural integrity, folding, assembly and stability of S-crystallins. More importantly, we proposed that the perturbation of the dynamic equilibrium betweenS-crystallin oligomers was an important mechanism of congenital hereditary cataract. The selective stabilization of one specific high-order oligomer by mutations might also be deleterious to the stability and folding of the p-crystalllin homomers and heteromers. The long-term structural stability and functional maintenance of13crystallins are achieved by the precisely regulated oligomeric equilibrium. (C) 2013 Elsevier B.V. All rights reserved.
