Paper Citing NAMD - Abstract
Preller, Matthias; Holmes, Kenneth C.
The Myosin Start-of-Power Stroke State and How Actin Binding Drives the Power Stroke
CYTOSKELETON, 70:651-660, OCT 2013
We propose that on binding to actin at the start of the power stroke the myosin cross-bridge takes on the rigor configuration at the actin interface. Starting from the prepower stroke state, this can be achieved by a small movement (16 degrees rotation) of the lower 50K domain without twisting the central -sheet or opening switch-1 or switch-2. The movement of the lower 50K domain puts a strain on the W-helix. This strain tries to twist the -sheet, which could drive the power stroke. This would provide a coupling between actin binding and the execution of the power stroke. During the power stroke the -sheet twists, moving the P-loop away from switch-2, which opens the nucleotide binding pocket and separates ADP from P-i. The power stroke is different from the recovery stroke because the upper and lower 50K domains are tethered in the rigor configuration. (c) 2013 Wiley Periodicals, Inc.
