Paper Citing NAMD - Abstract
Gosselin-Badaroudine, Pascal; Moreau, Adrien; Delemotte, Lucie; Cens, Thierry; Collet, Claude; Rousset, Matthieu; Charnet, Pierre; Klein, Michael L.; Chahine, Mohamed
Characterization of the honeybee AmNa(V)1 channel and tools to assess the toxicity of insecticides
SCIENTIFIC REPORTS, 5 Art. No. 12475, JUL 23 2015
Pollination is important for both agriculture and biodiversity. For a significant number of plants, this process is highly, and sometimes exclusively, dependent on the pollination activity of honeybees. The large numbers of honeybee colony losses reported in recent years have been attributed to colony collapse disorder. Various hypotheses, including pesticide overuse, have been suggested to explain the disorder. Using the Xenopus oocytes expression system and two microelectrode voltageclamp, we report the functional expression and the molecular, biophysical, and pharmacological characterization of the western honeybee's sodium channel (Apis Mellifera Na(V)1). The Na(V)1 channel is the primary target for pyrethroid insecticides in insect pests. We further report that the honeybee's channel is also sensitive to permethrin and fenvalerate, respectively type I and type II pyrethroid insecticides. Molecular docking of these insecticides revealed a binding site that is similar to sites previously identified in other insects. We describe in vitro and in silico tools that can be used to test chemical compounds. Our findings could be used to assess the risks that current and next generation pesticides pose to honeybee populations.
