Paper Citing NAMD - Abstract
des Georges, Amedee; Hashem, Yaser; Unbehaun, Anett; Grassucci, Robert A.; Taylor, Derek; Hellen, Christopher U. T.; Pestova, Tatyana V.; Frank, Joachim
Structure of the mammalian ribosomal pre-termination complex associated with eRF1 center dot eRF3 center dot GDPNP
NUCLEIC ACIDS RESEARCH, 42:3409-3418, MAR 2014
Eukaryotic translation termination results from the complex functional interplay between two release factors, eRF1 and eRF3, in which GTP hydrolysis by eRF3 couples codon recognition with peptidyl-tRNA hydrolysis by eRF1. Here, we present a cryoelectron microscopy structure of pre-termination complexes associated with eRF1 center dot eRF3 center dot GDPNP at 9.7-angstrom resolution, which corresponds to the initial pre-GTP hydrolysis stage of factor attachment and stop codon recognition. It reveals the ribosomal positions of eRFs and provides insights into the mechanisms of stop codon recognition and triggering of eRF3's GTPase activity.
