Shim, Joong-Youn; Ahn, Kwang H.; Kendall, Debra A.
Molecular Basis of Cannabinoid CB1 Receptor Coupling to the G Protein Heterotrimer G alpha(i)beta gamma IDENTIFICATION OF KEY CB1 CONTACTS WITH THE C-TERMINAL HELIX alpha(5) OF G alpha(i)
JOURNAL OF BIOLOGICAL CHEMISTRY, 288:32449-32465, NOV 8 2013

The cannabinoid (CB1) receptor is a member of the rhodopsin-like G protein-coupled receptor superfamily. The human CB1 receptor, which is among the most expressed receptors in the brain, has been implicated in several disease states, including drug addiction, anxiety, depression, obesity, and chronic pain. Different classes of CB1 agonists evoke signaling pathways through the activation of specific subtypes of G proteins. The molecular basis of CB1 receptor coupling to its cognate G protein is unknown. As a first step toward understanding CB1 receptor-mediated G protein signaling, we have constructed a ternary complex structural model of the CB1 receptor and G(i) heterotrimer (CB1-G(i)), guided by the x-ray structure of (2)-adrenergic receptor ((2)AR) in complex with G(s) ((2)AR-G(s)), through 824-ns duration molecular dynamics simulations in a fully hydrated 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine bilayer environment. We identified a group of residues at the juxtamembrane regions of the intracellular loops 2 and 3 (IC2 and IC3) of the CB1 receptor, including Ile-218(3.54), Tyr-224(IC2), Asp-338(6.30), Arg-340(6.32), Leu-341(6.33), and Thr-344(6.36), as potential key contacts with the extreme C-terminal helix (5) of G(i). Ala mutations of these residues at the receptor-G(i) interface resulted in little G protein coupling activity, consistent with the present model of the CB1-G(i) complex, which suggests tight interactions between CB1 and the extreme C-terminal helix (5) of G(i). The model also suggests that unique conformational changes in the extreme C-terminal helix (5) of G play a crucial role in the receptor-mediated G protein activation.

DOI:10.1074/jbc.M113.489153

Find full text with Google Scholar.