Paper Citing NAMD - Abstract
Joram Castro, Josue; Pablo Mendez, Juan; Mauricio Coral-Vazquez, Ramon; Antonio Soriano-Ursua, Marvin; Damian-Matsumura, Pablo; Benitez-Granados, Jesus; Rosas-Vargas, Haydee; Canto, Patricia
In Vitro and Molecular Modeling Analysis of Two Mutant Desert Hedgehog Proteins Associated with 46,XY Gonadal Dysgenesis
DNA AND CELL BIOLOGY, 32:524-530, SEP 2013
Mutations of Desert hedgehog (DHH) have been associated to 46,XY pure gonadal dysgenesis (PGD) and to mixed gonadal dysgenesis (MGD); however, there have been no functional studies of mutations described in DHH. To determine if mutations p.L162P and D1086delG yield functional impairment, we performed in vitro and in silico analysis of both DHH mutants. In complementary DNA of DHH, we performed site-directed mutagenesis, which was confirmed by DNA sequencing. Protein extracts were obtained from HEK293cells transfected with different constructs and analyzed by Western blot; besides, densitometric analysis of chemiluminescent signals was performed. In addition, the structure of the wt-DHH and its two mutant proteins was inferred using in silico protein molecular modeling. In the Western blot analysis, we observed the absence of signal for p. L162P in DHH-N and a diminished signal for D1086delG in DHH-C, when compared to wt-DHH. Protein modeling showed notable conformational changes for the side chains of p. L162P, while the secondary structure was drastically modified in D1086delG, when compared to wt-DHH. To our knowledge, this is the first study focused to determine by in vitro studies, the effect of two specific mutations in DHH associated with 46, XY PGD and MGD. Our results suggest that both mutations have a deleterious effect on the expression of the DHH mutant proteins.
