Paper Citing NAMD - Abstract
Chen, Rong; Chung, Shin-Ho
Complex Structures between the N-Type Calcium Channel (Ca(V)2.2) and omega-Conotoxin GVIA Predicted via Molecular Dynamics
BIOCHEMISTRY, 52:3765-3772, MAY 28 2013
The N-type voltage-gated Ca2+ channel Ca(V)2.2 is one of the important targets for pain management. omega-Conotoxins isolated from venoms of cone snails, which specifically inhibit Ca(V)2.2, are promising scaffolds for novel analgesics. The inhibitory action of omega-conotoxins on Ca(V)2.2 has been examined experimentally, but the modes of binding of the toxins to this and other related subfamilies of Ca2+ channels are not understood in detail. Here molecular dynamics simulations are used to construct models of omega-conotoxin GVIA in complex with a homology model of the pore domain of Ca(V)2.2. Three different binding modes in which the side chain of Lys2, Arg17, or Lys24 from the toxin protrudes into the selectivity filter of Ca(V)2.2 are considered. In all the modes, the toxin forms a salt bridge with an aspartate residue of subunit II just above the EEEE ring of the selectivity filter. Using the umbrella sampling technique and potential of mean force calculations, the half-maximal inhibitory concentration (IC50) values are calculated to be 1.5 and 0.7 nM for the modes in which Lys2 and Arg17 occlude the ion conduction pathway, respectively. Both IC50 values compare favorably with the values of 0.04-1.0 nM determined experimentally. The similar IC50 values calculated for the different binding modes demonstrate that GVIA can inhibit Ca(V)2.2 with alternative binding modes. Such a multiple-binding mode mechanism may be common for omega-conotoxins.
