Paper Citing NAMD - Abstract
Ling, Zhenmin; Liu, Yi; Teng, Shaolei; Kang, Zhen; Zhang, Jingjing; Chen, Jian; Du, Guocheng
Rational Design of a Novel Propeptide for Improving Active Production of Streptomyces griseus Trypsin in Pichia pastoris
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79:3851-3855, JUN 2013
Applying in silico simulations and in vitro experiments, the amino acid proline was proved to have a profound influence on Streptomyces griseus trypsinogen, and the hydrogen bond between H-57 and D-102 was found to be crucial for trypsin activity. By introducing an artificial propeptide, IVEF, the titer of trypsin was increased 6.71-fold.
