Paper Citing NAMD - Abstract
Zhou, Andy; Abu-Baker, Shadi; Sahu, Indra D.; Liu, Lishan; McCarrick, Robert M.; Dabney-Smith, Carole; Lorigan, Gary A.
Determining alpha-Helical and beta-Sheet Secondary Structures via Pulsed Electron Spin Resonance Spectroscopy
BIOCHEMISTRY, 51:7417-7419, SEP 25 2012
A new method has been developed to determine alpha-helical and beta-sheet secondary structural components of aqueous and membrane-bound proteins using pulsed electron paramagnetic resonance (EPR) spectroscopy. The three-pulse electron spin echo envelope modulation (ESEEM) technique was used to detect weakly coupled H-2-labeled nuclei on side chains in the proximity of a strategically placed nitroxide spin-label up to 8 angstrom away. Changes in the ESEEM spectra for different samples correlate directly to periodic structural differences between alpha-helical and beta-sheet motifs. These distinct trends were demonstrated with alpha-helical (M2 delta subunit of the acetylcholine receptor) and beta-sheet (ubiquitin) peptides in biologically relevant sample environments.
