Paper Citing NAMD - Abstract
Cueva, Juan G.; Hsin, Jen; Huang, Kerwyn Casey; Goodman, Miriam B.
Posttranslational Acetylation of alpha-Tubulin Constrains Protofilament Number in Native Microtubules
CURRENT BIOLOGY, 22:1066-1074, JUN 19 2012
Background: Microtubules are built from linear polymers of alpha-beta tubulin dimers (protofilaments) that form a tubular quinary structure. Microtubules assembled from purified tubulin in vitro contain between 10 and 16 protofilaments; however, such structural polymorphisms are not found in cells. This discrepancy implies that factors other than tubulin constrain microtubule protofilament number, but the nature of these constraints is unknown. Results: Here, we show that acetylation of MEC-12 alpha-tubulin constrains protofilament number in C. elegans touch receptor neurons (TRNs). Whereas the sensory dendrite of wildtype TRNs is packed with a cross-linked bundle of long, 15-protofilament microtubules, mec-17;atat-2 mutants lacking alpha-tubulin acetyltransferase activity have short microtubules, rampant lattice defects, and variable protofilament number both between and within microtubules. All-atom molecular dynamics simulations suggest a model in which acetylation of lysine 40 promotes the formation of interprotofilament salt bridges, stabilizing lateral interactions between protofilaments and constraining quinary structure to produce stable, structurally uniform microtubules in vivo. Conclusions: Acetylation of alpha-tubulin is an essential constraint on protofilament number in vivo. We propose a structural model in which this posttranslational modification promotes the formation of lateral salt bridges that fine-tune the association between adjacent protofilaments and enable the formation of uniform microtubule populations in vivo.
