Paper Citing NAMD - Abstract
Janovec, Ladislav; Kozurkova, Maria; Sabolova, Danica; Ungvarsky, Jan; Paulikova, Helena; Plsikova, Jana; Vantova, Zuzana; Imrich, Jan
Cytotoxic 3,6-bis((imidazolidinone)imino)acridines: Synthesis, DNA binding and molecular modeling
BIOORGANIC & MEDICINAL CHEMISTRY, 19:1790-1801, MAR 1 2011
New acridine derivatives bearing two symmetrical imidazolidinone rings, 3,6-bis((1-alkyl-5-oxo-imidazolidin-2-yliden)imino)acridine hydrochlorides 6a-6e (alkyl = ethyl, n-propyl, n-butyl, n-pentyl, n-hexyl), have been prepared and their interactions with calf thymus DNA and selected cell lines were studied. The DNA-binding of 6a-6e to ctDNA was examined by UV-vis, fluorescence, and CD spectroscopy. The binding constants determined by UV-vis spectroscopy were found in the range 1.9 x 10(5)-7.1 x 10(5) M(-1). An electrophoretic separation proved that ligands 6a-6e inhibited topoisomerase I in 40 mu M concentration although only those with longer alkyl chains were able to penetrate the membranes and efficiently suppress the cell proliferation. The highest activity in cytotoxic tests was found for 3,6-bis((1-n-hexyl-5-oxo-imidazolidin-2-yliden) imino) acridine hydrochloride (6e) with IC(50) = 2.12 mu M (HL 60) and 5.28 mu M (L1210) after 72 h incubation. Molecular dynamics simulations and calculations of solvent-accessible surface areas (SASAs) were used to explore the intercalation mechanism. MD simulations favor stacking between adjacent C: G base pairs from the minor groove side. MD and SASAs calculations indicate that the decrease of K with alkyl extension is due to negative entropic change upon binding. (C) 2011 Elsevier Ltd. All rights reserved.
