TCB Publications - Abstract
Thomas W. Lynch, Mark A. McLean, Dorina Kosztin, Klaus Schulten, and Stephen G. Sligar. High pressure gel mobility shift analysis and molecular dynamics: Investigating specific protein-nucleic acid recognition. In R. Hayashi, editor, Trends in High Pressure Bioscience and Biotechnology, pp. 87-94. Elsevier, 2002.
) at elevated pressures. From the dependence of on pressure, the volume change (
) of dissociation was determined. Molecular Dynamic (MD) simulations on the BamHI-DNA complex at both ambient and elevated pressures were performed to identify the structural origins of the observed experimental results. The simulation trajectories have identified important protein-DNA recognition elements that are disrupted with pressure. The trajectories also illustrate an increased hydration of the BamHI-DNA interface at elevated pressure. Both of these calculated pressure effects would favor dissociation of the complex. The combination of MD simulations and high pressure gel shift analysis proved useful in identifying thesse factors for maintaining BamHI-DNA complex stability. Download Full Text
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