TCB Publications - Abstract

Xabier Agirrezabala, Hstau Liao, Eduard Schreiner, Jie Fu, Rodrigo Ortiz-Meoz, Klaus Schulten, Rachel Green, and Joachim Frank. Structural characterization of mRNA-tRNA translocation intermediates. Proceedings of the National Academy of Sciences, USA, 109:6094-6099, 2012. (PMC: 3340995)

AGIR2012 Analysis of a wild-type E. coli pre-translocational sample competent for unimpaired forward translocation by cryo-EM, classification, and single-particle reconstruction has revealed the presence of previously unseen intermediate substates of the bacterial ribosome during the first phase of translocation, characterized by intermediate intersubunit rotations, L1 stalk positions, and tRNA configurations. Furthermore, we have described the domain rearrangements in quantitative terms, which has allowed us to define the processivity and coordination of the conformational reorganization of the ribosome, along with the associated changes in tRNA ribosome-binding configuration. The observed occupancies of substates also allowed a profile of the free-energy landscape to be reconstructed. The results are consistent with the view of the ribosome as a molecular machine employing Brownian motion to reach a functionally productive state (“macrostate II”) via a series of substates with incremental changes in conformation.


Download Full Text

The manuscripts available on our site are provided for your personal use only and may not be retransmitted or redistributed without written permissions from the paper's publisher and author. You may not upload any of this site's material to any public server, on-line service, network, or bulletin board without prior written permission from the publisher and author. You may not make copies for any commercial purpose. Reproduction or storage of materials retrieved from this web site is subject to the U.S. Copyright Act of 1976, Title 17 U.S.C.

Download full text: Journal, Request a Copy, Supplemental Material ( 6.4MB) - Supplementary PDF, Supplemental Material ( 4.8MB) - Supplementary movie 1, Supplemental Material ( 4.8MB) - Supplementary movie 2