Paper Citing NAMD - Abstract
Korchowiec, Beata; Korchowiec, Jacek; Hato, Masakatsu; Rogalska, Ewa
Glycolipid-cholesterol monolayers: Towards a better understanding of the interaction between the membrane components
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1808:2466-2476, OCT 2011
In this work, the interaction between a synthetic analog of archaeal lipids and cholesterol was studied using Langmuir technique. The lipid, beta-Mal(3)O(C16+ 4)(2), contained phytanyl chains attached via two ether bonds to the sn-2 carbon of the glycerol backbone. The preliminary studies showed that monolayers formed with the pure lipid have a liquid-like character: here, a hypothesis that admixing cholesterol to beta-Mal(3)O(C16+4)(2) could confer a higher rigidity on the films was tested. To check this proposal, two-dimensional miscibility of cholesterol and beta-Mal(3)O(C16+4)(2) in monomolecular films was studied using surface pressure and surface potential measurements, as well as Brewster angle microscopy and polarization-modulation infrared reflection absorption spectroscopy. The stability of the monomolecular films was evaluated based on thermodynamics of mixing of cholesterol and beta-Mal(3)O(C16+4)(2). Atomic level information concerning the orientation of molecules and the degree of hydration of polar headgroups was obtained from molecular dynamics simulations. (C) 2011 Elsevier B.V. All rights reserved.
