Paper Citing NAMD - Abstract
Trabuco, Leonardo G.; Schreiner, Eduard; Eargle, John; Cornish, Peter; Ha, Taekjip; Luthey-Schulten, Zaida; Schulten, Klaus
The Role of L1 Stalk-tRNA Interaction in the Ribosome Elongation Cycle
JOURNAL OF MOLECULAR BIOLOGY, 402:741-760, OCT 1 2010
The ribosomal L1 stalk is a mobile structure implicated in directing tRNA movement during translocation through the ribosome. This article investigates three aspects of L1 stalk tRNA interaction. First, by combining data from cryo electron microscopy, X-ray crystallography, and molecular dynamics simulations through the molecular dynamics flexible fitting method, we obtained atomic models of different tRNAs occupying the hybrid P/E state interacting with the L1 stalk. These models confirm the assignment of fluorescence resonance energy transfer states from previous single-molecule investigations of L1 stalk dynamics. Second, the models reconcile how initiator tRNA(fmet) interacts less strongly with the L1 stalk compared to elongator tRNA(Phe), as seen in previous single-molecule experiments. Third, results from a simulation of the entire ribosome in which the L1 stalk is moved from a half-closed conformation to its open conformation are found to support the hypothesis that L1 stalk opening is involved in tRNA release from the ribosome. (C) 2010 Elsevier Ltd. All rights reserved.
