Paper Citing NAMD - Abstract
Barakat, Khaled; Mane, Jonathan; Friesen, Douglas; Tuszynski, Jack
Ensemble-based virtual screening reveals dual-inhibitors for the p53-MDM2/MDMX interactions
JOURNAL OF MOLECULAR GRAPHICS & MODELLING, 28:555-568, FEB 26 2010
The p53 protein, a guardian of the genome, is inactivated by mutations or deletions in approximately half of human tumors. While in the rest of human tumors, p53 is expressed in wild-type form, yet it is inhibited by over-expression of its cellular regulators MDM2 and MDMX Proteins. Although the p53-binding sites within the MDMX and MDM2 Proteins are closely related, known MDM2 small-molecule inhibitors have been shown experimentally not to bind to its homolog, MDMX As a result, the activity of these inhibitors including Nutlin3 is compromised in tumor cells over-expressing MDMX, preventing these compounds from fully activating the p53 protein. Here, we applied the relaxed complex scheme (RCS) to allow for the full receptor flexibility in screening for dual-inhibitors that can Mutually antagonize the two p53-regulator proteins. First, we filtered the NCI diversity set, DrugBank compounds and a derivative library for MDM2-inhibitors against 28 dominant MDM2-conformations. Then, we screened the MDM2 top hits against the binding site of p53 within the MDMX target. Results described herein identify a set Of Compounds that have been computationally predicted to ultimately activate the p53 pathway in tumor cells retaining the wild-type protein. Crown Copyright (C) 2009 Published by Elsevier Inc. All rights reserved
