Paper Citing NAMD - Abstract
Fadouloglou, Vasiliki E.; Bastaki, Marina N.; Ashcroft, Alison E.; Phillips, Simon E. V.; Panopoulos, Nicholas J.; Glykos, Nicholas M.; Kokkinidis, Michael
On the quaternary association of the type III secretion system HrcQ(B)-C protein: Experimental evidence differentiates among the various oligomerization models
JOURNAL OF STRUCTURAL BIOLOGY, 166:214-225, MAY 2009
The HrcQ(B) protein from the plant pathogen Pseudomonas syringae is a core component of the bacterial type III secretion apparatus. The core consists of nine proteins widely conserved among animal and plant pathogens which also share sequence and structural similarities with proteins from the bacterial flagellum. Previous studies of the carboxy-terminal domain of HrcQ(B) (HrcQ(B)-C) and its flagellar homologue, FliN-C, have revealed extensive sequence and structural homologies, similar subcellular localization, and participation in analogous protein-protein interaction networks. It is not clear however whether the similarities between the two proteins extend to the level of quaternary association which is essential for the formation of higher-order structures within the TTSS. Even though the crystal structure of the FliN is a dimer, more detailed studies support a tetrameric donut-like association. However, both models, dimer and donut-like tetramer, are quite different from the crystallographic elongated dimer of dimers of the HrcQ(B)-C. To resolve this discrepancy we performed a multidisciplinary investigation of the quaternary association of the HrcQ(B)-C, including mass-spectrometry, electrophoresis in non-reductive conditions, gel filtration, glutaraldehyde cross-linking and small angle X-ray scattering. Our experiments indicate that stable tetramers of elongated shape are assembled in solution, in agreement with the results of crystallographic studies. Circular dichroism data are consistent with a dimer-dimer interface analogous to the one established in the crystal structure. Finally, molecular dynamics simulations reveal the relative orientation of the dimers forming the tetramers and the possible differences from that of the crystal structure. (C) 2009 Published by Elsevier Inc.
