TCB Publications - Abstract

TCB Publications - Abstract

T. Vosegaard, K. Bertelsen, J. M. Pedresen, L. Thøgersen, B. Schiøtt, E. Tajkhorshid, T Skrydstrup, and N. Chr. Nielsen. Resolution enhancement in solid-state NMR spectra of oriented membrane proteins by anisotropic differential linebroadening. Journal of the American Chemical Society, 130:5028-5029, 2008.

VOSE2008-ET We demonstrate that a significant improvement in the spectral resolution may be achieved in solid-state NMR experiments of proteins in inhomogeneously disordered oriented lipid bilayers. Using 1H homonuclear decoupling instead of standard $^1$H heteronuclear decoupling, the $^{15}$N line widths may be reduced by up to seven times for such samples. For large oriented membrane proteins, such resolution enhancements may be crucial for assignment and structural interpretation.

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