TCB Publications - Abstract

TCB Publications - Abstract

Timothy A. Isgro and Klaus Schulten. Binding dynamics of isolated nucleoporin repeat regions to importin-β. Structure, 13:1869-1879, 2005.

ISGR2005 The nuclear pore complex, through the interaction of its proteins with transport receptors, controls the transport of large molecules into and out of the cell's nucleus. Ample evidence exists for an essential role of proteins with FG sequence repeats in this control. Previous studies have elucidated binding spots for FG sequence repeats on the surface of the transport receptor importin-$\beta$ by X-ray crystallography and mutational studies. Molecular dynamics simulations have been performed to characterize the interaction of FG sequence repeats with the transport receptor. Observed binding spots have been verified and novel sites discovered, suggesting that importin-$\beta$ features many more binding spots than suspected so far. The observed binding spots are in accord with several models of nucleocytoplasmic transport and the large number of binding spots on importin-$\beta$ may be necessary for the pore complex to distinguish between importin-$\beta$ and inert proteins and allow it passage through the pore.

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