Amber topology for mutation

From: Aashish Bhatt (aashish.ph16221_at_inst.ac.in)
Date: Mon Sep 16 2019 - 10:40:50 CDT

Dear Sir

I have followed the tutorial "*A Tutorial on Alchemical Free Energy
Perturbation Calculations in NAMD" section 3 :**Mutation of tyrosine into
alanine"*
I have trying to calculate free energy perturbation for mutating protein.
My main problem is i am using amber topology.
Is there any alternative for this methodology to be used in amber format?

Best Regards
Aashish

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