Re: capping by psfgen

From: Brian Bennion (brian_at_youkai.llnl.gov)
Date: Mon Apr 05 2004 - 11:59:38 CDT

Hello Gavin,

I don't know if anyone else has replied, but adding the command

first none

in your segment build will stop psfgen from patching the n-terminus.

Brian

On Sun, 4 Apr 2004 tsaih_at_ncifcrf.gov wrote:

>
> Hi Everyone,
>
> I tried to cap my peptide by Ace at the N-terminii.
> I used "patch ACE FRAG:1". The ACE is patched to my peptide. Nevertheless,
> the psfgen also automatically adds the normal NH3+ to the first residue.
> How can I solve problem? any hint is welcomed! Many thanks!
>
> Gavin
>
> -------------------------------------------------
>

*****************************************************************
**Brian Bennion, Ph.D. **
**Computational and Systems Biology Division **
**Biology and Biotechnology Research Program **
**Lawrence Livermore National Laboratory **
**P.O. Box 808, L-448 bennion1_at_llnl.gov **
**7000 East Avenue phone: (925) 422-5722 **
**Livermore, CA 94550 fax: (925) 424-6605 **
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