Contents

• Introduction to Aquaporin Structure
  • Structure and Function of Aquaporins
  • Loading other aquaporin structures
  
  
• Structural Alignment of Aquaporins
  • Starting Multiple Sequence Alignment
  • Setting parameters for Multiple Structure Alignments
  • Aligning the molecules
  
  
• Comparing Protein Sequence and Structure
  • Protein Structure
  • Protein Sequence
  
  
• Residue Selection
  • Structure conservation
  • Sequence conservation
  
  
• Investigating Structural Alignment
  • RMSD per Residue
  
  
• Examining the Aquaporin Tetramer
  • Loading Tetramer
  • Examining channel lining and tetrameric contacts
  
  
• Phylogenetic Tree
• Evolutionary Profile of AQPs
  • Configure BLAST for Multiseq
  • Load Structures for AQPs in all three domains of life
  • Load Sequences for AQPs in all three domains of life
  • Align Sequences Using a Structural Profile
  • Construct an Evolutionary Profile for AQPs