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Introduction
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Rosetta/MDFF Tutorial
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Rosetta/MDFF Tutorial
Contents
Introduction
Required software
ModelMaker
Rosetta
MODELLER
Folding protein termini using ModelMaker
Structure prediction
Interactive fitting to a cryo-EM density with iMDFF and QwikMD
Modeling amino acid insertions
Structure prediction with Rosetta
Interactive fitting to a cryo-EM density with iMDFF
Model fit to mid-resolution EM density
MDFF run
Cross correlation coloring
Mid-resolution refinement
Structure check
Homology model
Building a homology model with MODELLER
Rigid body docking to the Rpn11 human density
Interactive MDFF run to the human Rpn11 density
High-resolution real-space refinement
www.ks.uiuc.edu/Training/Tutorials