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AARSs in Methanococcus jannaschii
In this section, you will find all the tRNA synthetases in an
organism. The M. janaschii genome [5], with NCBI
accession number NC_000909, has been completely sequenced, so we can
perform comprehensive searches through all of its genes.
- Open the Mozilla web browser: type mozilla at a command
line or click on the appropriate icon.
- Access the NCBI database (http://www.ncbi.nlm.nih.gov).
- Type the M. jannaschii accession number NC_000909 into the Search box, with
Genome selected as the search type, click on the single result, and
you will reach the site with the complete annotated sequence of M.
jannaschii.
- The best way to find Class II tRNA synthetases in this genome is via the listing of proteins organized by COG (Cluster of Orthologous Groups) functional categories. Click on the COG functional categories link located about halfway down the page as part of the text ``Gene Classifaction based on COG functional categories'' (note: this is not the COGs link, located near the top of the page, just beneath ``BLAST protein homologs''). You will reach a catalog of M. jannaschii proteins organized by functional annotation.
- Choose the link which it most likely to provide you with information about class II tRNA synthetases. (Hint: tRNA synthetase function is part of translation.)
Click on this link and scroll to the search fields at the bottom of the
resulting page.
- Type the string tRNA synthetase in the text search box
and you will receive a summary of all tRNA synthetases in M. jannaschii.
Consult the genetic code in Table 2 for help in answering the following questions.
Next: Domain structure of class
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