forming disulfide after equilibrating system

From: dora guzman (
Date: Fri Dec 22 2006 - 17:24:10 CST

I hope someone can help me with this:

I have equilibrated my protein in reduced form (free cysteines), but I'm
wondering if there is a way to covalently restrain the atoms to form a
disulfide bridge at this point? Or do I need to start from scratch? I'd
like to perform SMD on both the oxidized and reduced forms of the protein.

Thanks in advance!


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