histidine protonation

From: xiaojing gong (gongxiaojing1981_at_yahoo.com.cn)
Date: Thu Nov 02 2006 - 08:18:27 CST

Dear all:
           I am confused that how to get the protonation state of HIS. I see that there are three types for me to choose. But, in my opinion, if the HIS is protoned,the net charge of HIS is not zero, how to measure the net charge and I wonder to know if I need to add something in my Nacl solution to get the HIS protoned.
          I will appreciate it if you can give me some suggestions.


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