From: Dhiraj Srivastava (dhirajks_at_gmail.com)
Date: Tue Aug 15 2006 - 11:53:47 CDT
hi all
i am trying to apply n terminal patch on proline so that the N atom
is not protonated and is neutral. this proline is not in polypeptide chain
but is as a substrate of my enzyme. i have tried several times but i am not
able to get a proline molecule with neutral amino group and a charged
carboxylate group. how can i do that. proline binds to my protein in
its -vely charged state and not in its zwiterionic form.
-- Dhiraj Kumar Srivastava Department of Chemistry University of Missouri-Columbia MO, 65211 Ph. no. 001-573-639-0153
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