protonation of some histidine residues

From: mdametto_at_mail.usf.edu
Date: Fri Sep 14 2007 - 16:05:53 CDT

Dear users,

I've been trying to protonate 3 histidines residues in a protein. I could
protonate all ASP and GLU residues using "patch" before, but I'm have
problem with the histidines. The error message that I got is:
"
applying patch HSP to 1 residues
unknown patch type HSP
"
Below is the script that I've been using. Please, any help is welcome.
Thank you very much.

topology top_all22_prot.inp
alias residue HIS HSE
alias atom ILE CD1 CD
alias atom GLY OXT OT1
segment prot { pdb prot_p.pdb }
coordpdb prot_p.pdb prot
patch HSP prot:36
patch HSP prot:81
patch HSP prot:116
guesscoord
writepdb prot_p1.pdb
writepsf prot_p1.psf

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